WebOct 30, 2015 · Anti-RNA blotting with chemiluminescent detection. 30 October 2015. This protocol is for northern blotting/dot-blotting to detect specific RNAs via chemiluminescence, using biotinylated DNA oligo probes. Gels etc are optimized for detection of 0.5-4kb mRNA transcripts. Much of it is adapted from ULTRAhyb-Oligo and Brightstar BioDetect ... WebFeb 5, 2014 · To dissociate biotinylated nucleic acids from Streptavidin-Coupled Dynabeads®, incubate the beads in 95% formamide + 10mM EDTA, pH 8.2 for 5 …
RNA Antisense Purification (RAP): Experimental Protocols
WebFeb 2, 2024 · The biotinylated RNA bound to proteins was pulled down from the cell lysate by streptavidin agarose beads. RNA was detected by RT-PCR (Figure 3) and associated proteins by mass spectrometry (Figure 4). Our findings show that an RNA aptamer tag to RNA molecule is an effective method to explore the functional roles of RNA-protein … WebBiotinylated IRE RNA Control 12 9 6 3 0 Water 48 51 54 57 60 . Total Volume (µ. L) 60 60 60 60 60 . 4. Add 50µL of the Biotinylated IRE RNA Control stock solution into wells A1-A5 of a 96-well plate. 5. In a microcentrifuge tube, make a 2500-fold dilution of the test RNA labeling reaction in water to achieve a final song never loved you by alone
Methods to study RNA–protein interactions Nature …
WebOct 12, 2013 · Streptavidin pull-down of enzymatically biotinylated RNAs. 5′-Labeled RNA N 22 was biotinylated with E. coli PAP, and body-labeled RNA 1600 was biotinylated … WebBiotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus ... sneezing, and anorexia. 1 IAV is a single-stranded, negative-sense RNA virus that belongs to the family Orthomyxoviridae. The genome of IAV is composed of eight segments that encode for at least 10–14 proteins, whereby the ... Webcan be biotinylated in bacterial, yeast, insect, and mammalian cells (7–11). Biotinylation can occur either by the cell’s endogenous ... BirA RNA expression by Northern blot analysis. A selected BirA pEV MEL clone was transfected with tagged GATA-1 pEV, and stable clones were double selected for puromycin and neomycin (Invitrogen). smallest paths in simple rectilinear polygons